ISHS Acta Horticulturae 459: International Symposium Brassica 97, Xth Crucifer Genetics Workshop
TRANSGENE EXPRESSION AND STABILITY IN BRASSICA |
| Authors: | P. Dale, N. Al-Kaff, A. Bavage, J. Irwin, I. Senior |
| Keywords: | Biosafety assessment, Brassica napus, Oilseed rape, Gene silencing, Transgene expression, Transformation |
| Abstract:
Transgenes are likely to be used widely for the development of new plant varieties for agriculture. An understanding of the causes of transgene stability and expression is important for various reasons: 1) to determine the utility of transgenes in agriculture; 2) for assessing their potential impacts on human health and the environment and; 3) to achieve a better basic understanding of the ways in which genes act and interact. Many companies and laboratories have experienced poor transgene expression, transgene instability and silencing. Transgene expression is influenced by various factors including: the nature of the construct; transformation events; background genotype; interactions with other transgenes; environmental conditions; and, in certain specific cases, by pathogen infection. Genetic homology between the transgene construct and other segments of nucleic acids within the cell can have a fundamental effect on the way transgenes work. It is, therefore, important to understand the underlying genetic mechanisms of these interactions. | |
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