ISHS Acta Horticulturae 459: International Symposium Brassica 97, Xth Crucifer Genetics Workshop
MARKER ASSISTED SELECTION OF LOW LINOLENIC ACID IN OILSEED SPECIES |
| Authors: | D.J. Somers, K.R.D. Friesen, G. Rakow |
| Keywords: | Brassica, RAPD, linolenic acid |
| Abstract:
Linolenic acid is a component of canola oil that is readily oxidized, imparting an undesirable flavor which reduces the shelf life and quality of canola oil. Inheritance of linolenic acid in seed oil is polygenic and linolenic acid content is strongly influenced by the environment. For these reasons, molecular markers are sought to assist in early and accurate selection of desired genotypes in breeding programs. Molecular markers associated with linolenic acid loci were identified in a doubled haploid population derived from Brassica napus lines, Apollo x YN90–1016 using RAPDs and bulk segregant analysis. A total of 13 markers were distributed over three linkage groups, which individually accounted for 29%, 13% and 5% of the phenotypic variation in linolenic acid content. The rapeseed fad3 gene was one of the 13 markers and mapped near the locus controlling 13% of the variation. The mode of inheritance appeared to be additive and a QTL analysis showed that collectively the three loci explained 51% of the phenotypic variation within this population. We identified PCR fragments for Apollo alleles (3% linolenic acid) at all three loci. Simultaneous selection for Apollo alleles at each locus resulted in a group of DH lines with 4.0% linolenic acid. | |
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