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ISHS Acta Horticulturae 459: International Symposium Brassica 97, Xth Crucifer Genetics Workshop

MAPPING THE RADISH INTROGRESSION CARRYING THE RFO RESTORER GENE USED FOR THE OGU-INRA CYTOPLASMIC MALE STERILITY IN RAPESEED (BRASSICA NAPUS L.)

Authors:   R. Delourme, R. Horvais, P. Barret, M. Renard, N. Foisset, G. Champagne, W.Y. Cheung, B.S. Landry
Keywords:   Brassica napus, Raphanus sativus, introgression, restorer gene, RAPD, RFLP
Abstract:
Bulked Segregant Analysis (BSA) and comparative mapping were applied to identify markers linked to the Rfo restorer gene used for the Ogu-INRA cytoplasmic male sterility in rapeseed. These markers were then used to localize the radish introgression on the B. napus genetic map and to assess the length of this introgression. The introgression mapped on DY15 linkage group and corresponded to around 50 cM of the B. napus genetic map. A QTL (Quantitative Trait Locus) involved in the variation of total seed glucosinolate content was located on this linkage group at a position corresponding to one end of the introgression.

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