ISHS Acta Horticulturae 457: Symposium on Plant Biotechnology as a tool for the Exploitation of Mountain Lands
ELM TISSUE CULTURE: MICROPROPAGATION OF CLONES RESISTANT TO DUTCH ELM DISEASE |
| Authors: | M. Anna, L. Mariella, M. Lorenzo |
| Abstract:
The genetic improvement has developed clones of hybrid elm resistant to the vascular wilt disease caused by Ophiostoma ulmi. The aims of this work were to highlight the morphogenetic ability of two hybrid elm clones (FL089 and FL094) and to establish an appropriate method for their vegetative propagation. The healthier procedures in the preparation of stock plants, the optimum concentration of sterilizing agent and time of sterilization were evalueted. Nodal segments were excised from soft green shoots and cultured on media with three different mineral compositions: LS, DKW, WPM. The effects of various amounts of growth regulators (0.25 and 0.50 mg/l BA; 0.2 and 0.4 mg/l IBA; 0.5 and 0.25 mg/l NAA;) were also investigated. Shoot proliferation was achieved in both clones on DKW medium without growth regulators whereas root formation was observed on FL094 and FL089 microcuttings cultured respectively on DKW and WPM media supplemented with the lowest concentration of the two auxins. Moreover, on these media, the elongation of the shoots allowed the successive multiplication by subculturing one-node microcuttings. The results revealed that the different genotype of the two hybrids affects the morphogenetic ability of the explants as regard the shoot and the root development. | |
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