ISHS Acta Horticulturae 454: III International Symposium on New Floricultural Crops
CONSTITUTIVE EXPRESSION OF β-GLUCURONIDASE GENE FUSED WITH STRESS-INDUCIBLE PROMOTER OF THE PATHOGENESIS-RELATED 1A PROTEIN GENE OF TOBACCO IN TRANSGENIC KALANCHOE BLOSSFELDIANA |
| Authors: | R. Aida, M. Shibata |
| Keywords: | genetic transformation |
| Abstract:
Pathogenesis-related 1a (PR1a) promoter derived from PR1a protein gene of tobacco has been reported to conduct stress-induced or salicylic acid (SA) -induced expression in tobacco, potato, lettuce and torenia. Constitutive expression, an abnormal response, of the promoter was reported only in an interspecific hybrid of Nicotiana glutinosa x Nicotiana debneyi. We report constitutive expression of ß-glucuronidase (GUS) gene fused with PR1a promoter in transgenic Kalanchoe blossfeldiana. The chimeric gene (PR1a/GUS) was introduced into K. blossfeldiana by Agrobacterium-mediated transformation. The expression level of GUS gene among 45 individual transformants were analyzed. The activity level in non-treated leaf of each plant was varied from near-zero to about 17000 nmoles 4 MU/mg protein/30min. Thus, several plants showed very high activity even at healthy (not-induced) condition. The activity level was almost same between non-treated and SA-treated leaf in most of transformants. These results suggested that the stress-inducible promoter of tobacco did not conduct its nature function but showed abnormal response, constitutive expression, in K. blossfeldiana. | |
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