ISHS Acta Horticulturae 454: III International Symposium on New Floricultural Crops
ANALYSIS OF THE PERICLINAL CHIMERIC STRUCTURE OF CHRYSANTHEMUM SPORTS BY RANDOM AMPLIFIED POLYMORPHIC DNA |
| Authors: | M. Shibata, S. Kishimoto, M. Hirai, R. Aida, I. Ikeda |
| Keywords: | random amplified polymorphic DNA (RAPD), periclinal chimera, chrysanthemum, sports, chromosome number |
| Abstract:
The periclinal chimeric structure of ‘Pink Marble’ and its 8 sports chrysanthemums (Dendranthema grandiflorum (Ramat.) Kitamura) was investigated by random amplified polymorphic DNA (RAPD). The plants derived from epidermal cells of disc florets were obtained by tissue culture from ‘Pink Marble’ and all 8 sports. Total DNA was extracted from leaves or roots and used for RAPD analysis. Three 12-mer random primers were selected out of 238 as discriminatory primers. DNA of root in sports series were used for the analysis of the inner corpus layer (L3). DNA of leaf of the plants induced by tissue culture was used for the analysis of the epidermal layer (L1). RAPD banding patterns confirmed that ‘Marble’ sports series have the periclinal chimeric structure. Relationship between RAPD pattern and loss in chromosomes was also discussed. | |
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