ISHS Acta Horticulturae 454: III International Symposium on New Floricultural Crops
INTROGRESSION IN JAPANESE EVERGREEN AZALEAS (RHODODENDRON KIUSIANUM AND R. KAEMPFERI) |
| Authors: | N. Kobayashi, S. Kawashima, T. Handa, K. Takayanagi, K. Arisumi |
| Keywords: | chloroplast DNA, rDNA, PCR, RFLP |
| Abstract:
Natural hybridization of Japanese evergreen azaleas has often generated morphological variations, which have been used for the parents of azalea breeding. We investigated wild populations of evergreen azaleas in Kyushu composed of Rhododendron kiusianum, R. kaempferi and their interspecific hybrids. Morphological characters such as leaf size and flower color clearly distinguished two species. PCR-RFLP analysis of cpDNA could detect their specific bands in 16S rDNA region digested with HhaI restriction enzyme. However, 24% of individuals, which were morphologically indistinguishable from R. kiusianum, possessed the cpDNA of R. kaempferi. This suggests that past introgression into R. kiusianum might have occurred. | |
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