ISHS Acta Horticulturae 451: VI International Symposium on Integrated Canopy, Rootstock, Environmental Physiology in Orchard Systems
A NEW IN VITRO METHODOLOGY FOR STUDYING CELL-CELL RECOGNITION IN PEAR-QUINCE GRAFT INCOMPATIBILITY |
| Authors: | S. Musacchi, M. Faust, J. Hartung, S. Sansavini |
| Keywords: | callus, SDS-PAGE, 2D-PAGE, protein analysis |
| Abstract:
Graft-incompatibility between pear (Pyrus communis) and quince (Cydonia oblonga) is a widespread phenomenon the causes of which are still poorly understood. Little information is available about cell-cell recognition and, although several theories about it have been propounded, none have established the existence of a recognition protein between the cells of the two genotypes in the graft union area. The present study reports and discusses an in vitro model system that was developed to identify proteins involved in pear-quince incompatibility based on growth patterns of the two genotypes merged in the same Petri dish. Calli from the pear cvs. ('Beurrè Hardy', 'Bartlett', 'Beurrè Bosc') and the rootstocks (Quince C, Quince BA29, Fox 11) were used. Measurements included scion callus growth as affected by rootstock and rootstock callus growth as affected by scion. Differences in the pear and quince protein expression were determined by SDS-PAGE and 2D-PAGE analysis. | |
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