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ISHS Acta Horticulturae 448: III International Symposium on Mineral Nutrition of Deciduous Fruit Trees

IDENTIFICATION OF DATE PALM CULTIVAR USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

Author:   N. Bouchireb
Abstract:
Eight cultivars of date pam from Algeria were differentiated using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). A set of 50 random sequence 10-mer primers were tested. A selection of six primers produced 31 polymorphic bands of between 300 to 1300 base pairs in size sufficient to distinguish between the cultivars. A combination of all the electrophoretic profiles allows a specific identification of each cultivar Genetic similarities between cultivars were calculated and cluster analysis was used to generate a dendogram showing the relationships between them. The variation detected between cultivars is not associated with their geographical repartition. RAPD technology provided a new alternative for cultivar identification.

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