IDENTIFICATION OF DATE PALM CULTIVAR USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)
Eight cultivars of date pam from Algeria were differentiated using randomly amplified polymorphic DNA (RAPD) markers obtained by the polymerase chain reaction (PCR). A set of 50 random sequence 10-mer primers were tested.
A selection of six primers produced 31 polymorphic bands of between 300 to 1300 base pairs in size sufficient to distinguish between the cultivars.
A combination of all the electrophoretic profiles allows a specific identification of each cultivar Genetic similarities between cultivars were calculated and cluster analysis was used to generate a dendogram showing the relationships between them.
The variation detected between cultivars is not associated with their geographical repartition.
RAPD technology provided a new alternative for cultivar identification.