ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
DIFFERENTIATION BETWEEN DIPLOID AND DOUBLE HAPLOID CHICORY (CICHORIUM INTYBUS L.) BY RAPD-PCR ANALYSIS |
| Authors: | R. Theiler, M. Lüscher, C. S. Hunter |
| Abstract:
Double haploid plants raised from microspore cultures of Witloof, Robin and Treviso chicory genotypes were subjected to DNA analysis by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) methods. Three out of thirty-nine 10mer primers tested led to polymorphic DNA-fragments by which the parent genotypes as well as microspore-derived plants therefrom were differentiated. Polymorphism of the RAPD-PCR-generated DNA fragment profiles ranged from high (different genotypes) to low (closely related genotypes) and monomorphic (cloned genotypes). This method enabled a clear distinction to be made between each parent genotype and their microspore-derived descendants, irrespective of their ploidy. | |
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