ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
INVESTIGATION OF MICROSPOROGENESIS IN GRAPE FOR DIHAPLOID PRODUCTION VIA ANTHER CULTURE |
| Authors: | R. Holló, S. Misik |
| Keywords: | Vitis, microscopy, carmine, hematoxylin, Alexander's method |
| Abstract:
Microsporogenesis of three Vitis varieties were investigated by light microscope. The traditional staining procedures, using carmine, hematoxylin, safranin, malachite green, were unsuccessful or took a long time. With the aid of the three-stain method of Alexander all of the developmental stages could be distinguished. This is the first step in our effort to produce dihaploid plants in grape | |
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