ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO ANDROGENESIS IN APPLE: OPTIMIZATION OF THE ANTHER CULTURE |
| Author: | M. Höfer |
| Keywords: | Malus x domestica, anther culture, embryogenesis, ploidy level |
| Abstract:
Status of haploid research in apple via anther culture and a protocol will be given. In apple in vitro androgenesis apple, embryoid induction, regeneration rate and the ploidy level of the embryoids are mainly determined by the genotype. By using different plant material from bud wood forced at different temperatures or from trees in the orchard, in combination with a cold pretreatment, embryoid induction frequency can remarkably improve in dependence on genotype. After transferring the androgenic embryoids to an optimized regeneration medium, a successful regeneration has been obtained by formation of adventitious shoots. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
