ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
PLANT REGENERATION VIA SOMATIC EMBRYOGENESIS IN GRAND NAIN BANANA AND ITS EFFECT ON SOMACLONAL VARIATION |
| Authors: | A. Shchukin, D. Ben-Bassat, Y. Israeli |
| Keywords: | Musa, Dicamba, semi-solid medium, off-types |
| Abstract:
The purpose of this study was to establish a simple and efficient procedure for inducing somatic embryogenesis (SE) and plant regeneration in Grand Nain banana and to evaluate the resultant rate of variation. Excised apices of in-vitro-cultured plants, including 3-5 leaf primordia and a few leaf bases, were grown on semi-solid SH (Schenk and Hildebrandt) medium supplemented with 2.2 mg.l-1 Thidiazuron (1-phenyl-3-(1,2,3-thidiazol-5-y) urea) and 5 or 7 mg.l-1 Dicamba (3,6-dichloro-2-methoxybenzoic acid). Embryogenic, creamy-white compact callus appeared after 3-6 months and its production peaked after 6–10 months. Plantlet regeneration was achieved by transplanting isolated embryogenic callus pieces onto semi-solid half-strength MS (Murashige and Skoog) basal medium supplemented with 5 mg.l-1 BAP (6-benzylaminopurine). Somatic embryos developed into small plantlets within 1–2 months. The plantlets were acclimated and transplanted into the field. When 5 mg.l-1 Dicamba was used in the induction medium, only 0.5% of 984 field-observed plants were off-types. When 7 mg.l-1 Dicamba was used, 3.6% off-types were recorded out of 1511 plants. Approximately 5.3% off-types were recorded among 2142 control plants, propagated concurrently via the common shoot-tip-culture procedure. We concluded that the rate of somaclonal variation in plants produced via SE is not higher than that in shoot-tip-propagated plants. | |
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