ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
TRANSFORMATION OF SOMATIC EMBRYOS OF VITIS SP. WITH DIFFERENT CONSTRUCTS CONTAINING NUCLEOTIDE SEQUENCES FROM NEPOVIRUS COAT PROTEIN GENES |
| Authors: | R. Gölles, R. Moser, H. Katinger, M. Laimer da Câmara Machado, V. Tsolova, A. da Câmara Machado, A. Bouquet |
| Abstract:
Grapevines, the world's most widely-grown fruit crop, are difficult to breed, as in the case with many woody crops, due to heterozygocity, long generation time, and cross incompatibility. Transformation offers new alternatives in the genetic improvement of grapevine. Nepoviruses are the grapevine viruses of greatest economic importance because of their worldwide occurrence and the severe damages they cause. An Agrobacterium-mediated gene transfer system relying on repetitive embryogenesis has been used to regenerate transgenic grapevines.
Embryogenic cultures of Vitis vinifera (Russalka - selfpollinated) and 110 Richter (Vitis rupestris x Vitis Berlandieri) were transformed with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene | |
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-glucuronidase (GUS), and seven different constructs containing chimeric coat protein (CP) genes of grapevine fanleaf virus (GFLV), including nontranslatable and truncated forms of the CP gene, and Arabis mosaic virus (ArMV), respectively.
Putative transformed embryos were selected by continued proliferation on kanamycin containing medium.
Embryos transformed with the plasmid pBinGUSint were shown to express the GUS gene by histochemical analyses.
