ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
HISTOGENESIS OF SOMATIC EMBRYOGENESIS IN ALSTROEMERIA L. |
| Authors: | C.E. Van Schaik, C. Van Der Toorn, M.J. De Jeu, E. Jacobsen |
| Keywords: | Alstroemeria, morphology, regeneration, origin |
| Abstract:
Immature zygotic embryos of a tetraploid Alstroemeria cultivar were cultured on callus induction medium to obtain embryogenic callus. This callus was transferred to regeneration medium in order to develop somatic embryos. Cultured tissue was sampled during callogenesis and somatic embryogenesis and prepared for light microscopical analysis. Histological studies of this tissue showed that the protoderm of the callus underwent repeated divisions. These cells became bulky with large vacuoles. Later on pro-embryos were observed between these bulky cells. Most somatic embryos had an unicellular origin or originated from a few cells. These somatic embryos revealed the typical features of zygotic Alstroemeria embryos having a cotyledon, root apex and a shoot apex in the germination notch. | |
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