ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
OCCURRENCE OF EMBRYO LIKE STRUCTURES AND PLANT REGENERATION FROM A CELL SUSPENSION OF LILIUM LONGIFLORUM |
| Authors: | A. Tribulato, P.C. Remotti, H.J.M. Löffler |
| Abstract:
Friable, embryogenic callus, from Easter lily 'Snow Queen' was obtained by culturing pistils and flower pedicels on MS medium supplemented with Dicamba. A fast growing cell line was established from small, friable callus segments incubated in liquid MS medium with 2μM Dicamba. From these cell suspension cultures embryo like structures (ELS) emerged. ELS were frequently attached to the callus clumps or floated freely in the medium. When placed on solidified, hormoneless MS medium, on filter paper the ELS germinated within a few days generating complete plantlets. | |
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