ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO PROPAGATION OF ANANAS THROUGH NODULE CULTURE |
| Authors: | W. Teng, L. Y.Y. Yu |
| Keywords: | bromeliad, direct regeneration, organogenesis |
| Abstract:
A propagation scheme for Ananas was developed using nodule culture. Nodules were induced by culturing either leaf base explants or chopped shoot base explants on modified half-strength MS medium supplemented with 2.69–5.37 μM NAA and 4.44 μM BA. The nodules proliferated more nodules when chopped into pieces of 1–3 mm and placed on the same medium. Nodules regenerated shoots when transferred to medium with 0.54–10.74 μM NAA and 0.44–8.88 μM BA. Maximum regeneration was obtained from 0.54–2.69 μM NAA and 0.44 μM BA, where shoots could be observed as early as within two weeks. Many shoots formed roots at the same medium where they were regenerated but the best rooting occurred at 0.54–2.69 μM NAA and 0.44 μM BA. By using nodules, numerous rooted plantlets of Ananas could be routinely produced at six-week intervals. | |
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