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ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding

IN VITRO PROPAGATION AND STUDY OF THE EPIDERMIS OF HOSTA FORTUNEI

Authors:   Z. Szafián, E. Jámbor-Benczúr, A. Csillag
Keywords:   6-benzylamino purine, naphtalaneacetic acid, kinetin, K-humate, acclimatization, in-vitro rooting
DOI:   10.17660/ActaHortic.1997.447.36
Abstract:
Plants were initiated from underground buds of Hosta fortunei 'Albopicta'. Shoots were cultured on a modified, half-strength Murashige-Skoog medium containing 1–6 mg/liter 6-benzylamino purine (BA) or kinetin (KIN) and 0,1 mg/l naphtalaneacetic acid (NAA). These plants could be separated and proliferated on a medium supplemented with 2 mg/liter BA and 0,1 mg/l NAA to gain large number of plants. These shoots were rooted on media contained growth regulators and other compounds (NAA, activated charcoal, K-humate). The basal media were modified MS (mMS) and S medium. The best root quality was on S medium containing 0.2 g/l K-humate and 0.1 mg/l NAA. Five months after transplanting to the soil no difference was observed between the plants. Regarding the study of the leaf epidermis, we concluded, that with the use of BA the cells of the upper side of the leaves were deformed much more than when with KIN. During the acclimatization, in the new leaves, cells regained their normal shape.

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