ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
THE EFFECT OF BASIC MEDIA AND GROWTH REGULATORS ON IN VITRO PROPAGATED SORBUS DEGENII 'CSÁKVÁR' |
| Authors: | A. Mándy, E. Jámbor-Benczúr, Z. Szafián, A. Csillag |
| Keywords: | micropropagation, thidiazuron, benzylamino-purine-ribosid, vitrification, silver-nitrate, SEM-examination |
| Abstract:
Different basic media and cytokinins were tested to obtain the greatest number of shoots in the best quality. The maximal shoot proliferation number was achieved with 0.5 mg/l TDZ (7,1 pieces), but those were not elongated and some of them were vitrified. With the use of BAR almost no vitrification occurred and the shoot proliferation rate exceeded 4 shoots per inoculum. 2 mg/l silver-nitrate mixed into the culture medium decreased the number of vitrificated plantlets. Also the cooling of the medium to 15 °C gave similar results. | |
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