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ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural  Breeding 

APPLICATION OF TISSUE CULTURE TO HORTICULTURE

Authors:   Trevor A. Thorpe, Indra S. Harry
Keywords:   anther/microspore culture, axillary bud breaking, embryo culture/rescue, germplasm storage, micropropagation, pathogen-free plants, plant modification, protoplasts, somaclonal variation, transformation
Abstract:
Tissue culture techniques have played an important role in the breeding, production and improvement of horticultural crops. The uses of embryo culture in fruit tree breeding and in the propagation of orchards in the 1930s were among the first applications of the technology to plants. The present role of tissue culture techniques in horticulture can be seen in the production of specific pathogen-free plants, germplasm storage, micropropagation and in plant modification. In plant modification and improvement, several different in vitro approaches are being used. These include embryo rescue, anther/microspore culture, somaclonal variation, protoplast fusion, and transformation. The utilization of these various techniques with suitable examples from fruit, vegetable and ornamental crops is discussed.

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