ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding
NEW SUBSTRATES AND PHYTOPROTECTION FOR SOMATIC EMBRYO GERMINATION AND DEVELOPMENT |
| Authors: | J.M. Dupuis, J.L. Arnault, E. Teufel |
| Abstract:
We have significantly improved the use of cellulose acetate as a substrate for somatic embryo development into plants. Used as a mini-plug, cellulose acetate produced conversion rates of somatic embryo to plantlets higher than 90%. Such mini-plugs may be partially covered on their outer surface by a film to control the water and nutrient supply. In such conditions, root emergence outside the coating was not inhibited by the presence of the film and emergence rates were identical to conversion rates (between 85 and 95% depending on the treatment). In addition, we have attempted to define a mixture of compounds with anti-microbial properties to ensure that problems do not arise during the sowing of the capsule or its delivery under non-sterile conditions. | |
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