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ISHS Acta Horticulturae 447: III International Symposium on In Vitro Culture and Horticultural Breeding

CYCLIC SOMATIC EMBRYOGENESIS OF ANTHURIUM SCHERZERIANUM SCHOTT.

Authors:   M. Hamidah, P. Debergh, A. Ghani, A. Karim
DOI:   10.17660/ActaHortic.1997.447.15
Abstract:
Primary somatic embryos were induced on leaf explants of Anthurium scherzerianum on modified MS medium. When primary embryos were isolated and recultured on induction medium stage 1, secondary embryogenesis occurred and this cycle could be repeated. The highest number of secondary embryos was obtained from mature and germinated embryos. No problems in embryo maturation and germination were encountered. Addition of absisic acid (ABA) did not influence maturation or shoot development. Liquid medium was more performant than solid. Fragmentation of somatic embryo clusters increased productivity. All shoots regenerated in all cycles were phenotypically normal.

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