ISHS Acta Horticulturae 444: III International Symposium on Kiwifruit
IN VIVO AND IN VITRO STUDIES OF A THERMOSTABLE NITRATE REDUCTASE IN ACTINIDIA |
| Authors: | C.J. Clark, E.A. Godlewska, H.L. Boldingh |
| Keywords: | enzyme, kiwifruit |
| Abstract:
In vivo and in vitro assays are described for the characterization of nitrate reductase in leaves, roots and fruit of kiwifruit (cv. 'Hayward'). A NADH/NADPH, bispecific nitrate reductase, stable for at least a month at 4 °C, and thermostable at temperatures to least 60 °C, has been identified in crude and partially-purified extracts of leaves and roots of kiwifruit. Stability characteristics were not confined to kiwifruit (Actinidia deliciosa) but were found to extend to other members of the genus also. The factors responsible for, or contributing to this stability are presently unknown. | |
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