Abstract:
Walnut is a popular and economically important species, but vegetative propagation by budding or cutting is quite difficult.
In this study propagation in vitro was tested.
Meristems, shoot tips, and axillary buds were taken from seedlings of J. regia 2 April 1993. Modified MS medium with 0.5 mg/L Benzyl amino purine (BAP) was used as shoot growing medium and l mg/L BAP as shoot poliferation medium.
The best growth in the growing medium was obtained from shoot tips (2–3 mm) and cuttings (2–2.5 cm) with 1–2 axillary buds.
The meristem is not a suitable explant for in vitro propagation of walnut when compared with shoot tips and axillary buds.
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