ISHS Acta Horticulturae 441: V Temperate Zone Fruit in the Tropics and Subtropics
CLONAL PROPAGATION OF PISTACIA ROOTSTOCKS BY MERISTEM AND SHOOT CULTURE |
| Authors: | S. Çetiner, H. Yücel, Y. Aka-Kaçar, Y. Yalçin-Mendi |
| Abstract:
The clonal propagation of rootstocks used for pistachio species such as Pistacia khinjuk Stock, Pistacia atlantica Desf., Pistacia terebintus L. and Pistacia integerrima Stewart via tissue culture techniques was investigated. Meristems and the shoots which were obtained by germinating the seeds were used as propagation materials. Different concentrations of BAP were used as cytokinin. Healthy shoot development was not obtained in Pistacia integerrima Stewart. In the other three species, shoot development was obtained in Murashige & Skoog medium containing 4 mg/l BAP, but the problems were not solved completely. Meristems of Pistacia terebintus L., Pistacia khinjuk Stock, and Pistacia atlantica Desf. showed 3.88, 2.20 and 2.20 multiplication ratio, respectively. The shoots of Pistacia terebintus L., Pistacia khinjuk Stock, Pistacia atlantica Desf. showed 1.97, 1.36 and 0.80 multiplication ratio, respectively. During in vitro propagation of Pistacia species, some problems, such as browning, death of shoot tips, vitrification, variation and callus production, were seen. In order to solve the problems which were encountered, some studies, such as subculturing frequently, usage of active charcoal, ascorbic acid, and citric acid, and changing media compositions were carried out. These problems were not eliminated totally, however. | |
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