ISHS Acta Horticulturae 441: V Temperate Zone Fruit in the Tropics and Subtropics
IN-VITRO MULTIPLICATION OF CLONAL APPLE ROOTSTOCKS M-9, M-26 AND MM-106 BY MERISTEM CULTURE |
| Authors: | K. Aklan, S. Çetiner, Y. Aka-Kaçar, Y. Yalçin-Mendi |
| Abstract:
Meristems of apple rootstocks M-9, M-26 and MM-106 were cultured on ½ strength MS medium containing 2.2 μM BA, 0.5 μM IBA, and 0.3 μM GA3. M-26 and MM-106 shoots were transferred on Lepoivre and MS media, respectively, supplemented with 4.4 μM BA, 5 μM IBA, 0.3 μM GA3. The rate of shoot proliferation per mother explant of M-26 and MM-106 were 6 and 3.4, respectively. The best rooting of MM-106 was achieved with the use of 4 μM IBA, 0.3 μM giberellic acid (GA3) and 1300 μM phloroglucinol (PG) in which 80% rooting was obtained. During the proliferation phase, shoot tip necrosis and vitrified shoots were observed with MM-106. To overcome these, PG was used. Although vitrification was not observed, the shoot elongation M-26 was limited to around 1 cm. In M-9, insufficient shoot proliferation was obtained, thus experiments are underway to increase shoot production. | |
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