ISHS Acta Horticulturae 440: International Symposium on Plant Production in Closed Ecosystems
GROWTH OF GERBERA HYBRIDA IN VITRO AS AFFECTED BY CO2 CONCENTRATION AND AIR EXCHANGE RATE OF THE VESSEL |
| Authors: | B.R. Jeong, C.S. Yang, J.C. Park |
| Keywords: | air exchange rate, CO2 concentration, gerbera, Gerbera hybrida, photoautotrophic |
| Abstract:
Growth of Gerbera hybrida Hort. cv. Ansofie plantlets as affected by three levels of CO2 concentration, 350–450 (ambient), 1,000, and 2,000 μmol mol-1, and two levels of number of air exchanges per hour, 0.5 and 2.0 h-1, was studied. Explants were obtained from photomixotrophically micropropagated plantlets and planted after removal of roots. Four explants were planted in each 4.0 x 10-4 m3 glass bottle with a clear plastic cap and containing 0.4 x 10-4 m3 MS agar medium with no added sugar. Explants were cultured for 35 days under 140 μmol m-2 s-1 PPF, provided by cool white fluorescent lamps for 16 h d-1, at 24±1°C air temperature and 70–80% RH. In treatments of 2.0 number of air exchanges per hour, a 10 mm round hole made on the vessel cap was sealed with a microporous filter. For higher CO2 concentration in the culture rooms, CO2 gas was provided from a liquefied CO2 tank. Fresh and dry weights, height, leaf area, and chlorophyll concentration significantly increased with increasing CO2 concentration. However, % dry matter and number of leaves were not affected by CO2 concentration. Chlorophyll concentration decreased significantly as air exchange rate increased. Fresh and dry weights, height, and leaf area significantly increased with, while % dry matter and number of leaves were not affected by, increasing air exchange rate. Fresh and dry weights, height, and leaf area were affected significantly by the interaction between CO2 concentration and air exchange rate of the culture vessel. Under 140 μmol m-2 s-1 PPF, 1,000 μmol mol-1 CO2 treatment gave the most vigorous growth of gerbera. Leaf yellowing was evident in the 2,000 μmol mol-1 CO2 treatment, and this seemed not to be caused by high PPF. | |
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