ISHS Acta Horticulturae 440: International Symposium on Plant Production in Closed Ecosystems
SEVERAL FACTORS AFFECTING BULBLET REGENERATION FROM THE CULTURE OF SCALE SEGMENT AND NODE-BUD IN FRITILLARY AS MEDICINAL BULBOUS PLANT |
| Authors: | K.Y. Paek, N.S. Sung, C.H. Park |
| Keywords: | Fritillaria spp, medicinal plant, micropropagation, node-bud, scale segment |
| Abstract:
Bulblet formation from cultured scale explants was promoted by dry storage of mother bulbs for 2 to 4 weeks at 4C or moisture storage for 4 to 6 weeks at 10C. Number of bulblets was significantly increased in the culture of young node-bud or stem explants compared to scale segments. Bubllet forming capacity in stem tissue was depended on physiological age of stem, younger stem had high regeneration ability compared to old stem taken from above 10 cm shoot length. Maximum multiplication rate of young stem and/or node-bud segments was about 20 times. Culture with rotary shaker at 100 rpm or rotation drum at 80 rpm had effective for the production of bulblets compare to those of reciprocal shaker. Scale leaf emergence from bulblets after transplanted into soil depended on chilling period, sucrose concentration during culture and bulblet size. Scale leaf emergence was stimulated by planting bigger size of bulblets which produced in 3% sucrose medium compared to small bulblet and/or bulblets obtained from medium containing more than 5% sucrose. | |
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