ISHS Acta Horticulturae 440: International Symposium on Plant Production in Closed Ecosystems
DIFFERENTIATION OF SPINACH PLANT (SPINACIA OLERACEA L.) DEVELOPMENT USING LASER INDUCED FLUORESCENCE (LIF) |
| Authors: | J. Norikane, T. Takakura, H. Tsuchiya, A. Yamazaki |
| Keywords: | chlorophyll fluorescence, laser induced fluorescence (LIF), spinach development |
| Abstract:
A laser induced fluorescence (LIF) system was used to determine spinach plant development from a distance of one meter. Spinach plants were divided into three groups whose ages were 32, 43 and 53 days old. The LIF system used an argon laser with an output wavelength of 488 nm and output power of 5 mW. A beam expanding lens was used to enlarge the irradiated area of the spinach leaf and produce an average chlorophyll fluorescence signal. A series of ten LIF measurements were taken at five second intervals of one leaf per plant. Chlorophyll a and b fluorescence peaks occurred at 685 nm and 735 nm, respectively. The ratio of chlorophyll a and chlorophyll b peaks decreased with plant age, from 1.28 to 1.15. This trend was due to the increase in chlorophyll content as the spinach plants matured. | |
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