ISHS Acta Horticulturae 440: International Symposium on Plant Production in Closed Ecosystems
MACHINE VISION SYSTEM FOR MEASUREMENT OF THE RATE OF JOINING OF GRAFTED SEEDLINGS |
| Authors: | T. Ten, Y. Nishiura, H. Murase, N. Honami, T. Taira, A. Wadano |
| Keywords: | grafting, grafting method, joining rate, 3-D image acquisition, vessel formation |
| Abstract:
Grafting is the technique where a cut scion and stock are joined together to produce a new plant. The scion transfers the energy produced by photosynthesis to all parts of the grafted plant. The stock supplies water and minerals to the scion where the photosynthesis takes place. To determine the rate of joining of the grafted plant direct measurement of the cross-section of the joint is taken. However, using this approach it is not possible to gather enough data for appropriately evaluating the conditions of the joint. Three grafting methods of 3-D image processing, Cutting, Cleft, and "Plug-in Method" grafting, were compared on the determination of the rate of grafting. The first two are manual and the last method is machine assisted. The system of study comprised of a microtome, a microscope, a CCD color camera and an image processor. 3-D images were obtained from the 2-D images. 3-D images were constructed to compare joint formation for seedlings grafted by "Plug-in Method", Cutting grafting and Cleft grafting. The 3-D method produced better images of the joints than those obtained from the 2-D method. Among the three grafting methods, the 3-D images for the "Plug-in Method" showed clear distinction in the development of vessels at the joint. From the results obtained it was found that the "Plug-in Method" grafting performed better than all the other two grafting methods and it shows good potential as a future grafting method. In particular the method can be used to examine the joining of vessels in relation to other physical activities such as water and nutrient supply when seedlings are grafted. | |
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