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ISHS Acta Horticulturae 440: International Symposium on Plant Production in Closed Ecosystems

IN VITRO PROPAGATION OF HOSTA USING CULTURED SHOOT TIPS AND SOMACLONAL VARIABILITY OF REGENERANTS

Authors:   K.Y. Paek, S.H. Ma
Keywords:   hosta, regenerant, shoot tip, somaclonal variant
DOI:   10.17660/ActaHortic.1996.440.101
Abstract:
The rate of axillary shoot multiplication was stimulated by the addition of either 0.01 or 0.10 mg/L alpha-naphthalenacetic acid to medium supplemented with 3.0 mg/L 6-benzylaminopurine. Indoleacetic acid and indolebutyric acid did not promote axillary and/or adventitious shoot formation compared to NAA treatment. BA stimulated a higher number of axillary shoots than 2iP and kinetin which are favored for adventitious shoot formation. Axillary and adventitious shoot multiplication was optimum under photosynthetically active radiation (PAR) of 70–80 μmol m-2s-1 at 25°C. Rooting of shoots in vitro was obtained on basal medium without growth regulators or on medium containing 0.01 mg/L NAA. Rate of shoot multiplication was greatly depended on the genotypes and chimeral or nonchimeral characteristics. The regenerants were successfully reestablished in soil. Some of the plants obtained from culture which had lost leaf marginal variegation regained it after one year culture in field condition. Frequencies of somaclonal variant plants produced from shoot tip culture ranged from 1.8% to 43.8%. Most genetic variants that occur in somatic tissue showed the difference of leaf area, chlorophyll content and petiole length compared to normal plants. Foliar variegation pattern and chimeral components of flower color pattern separated during regeneration.

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