ISHS Acta Horticulturae 440: International Symposium on Plant Production in Closed Ecosystems
EFFECT OF IN VITRO STORAGE CONDITIONS ON THE CONSUMPTION OF THE NUTRITIONAL CONTENTS AND THE VIABILITY OF LILIUM BULB-SCALE |
| Authors: | T. Yoshihara, F. Goto |
| Keywords: | bulblet regeneration, content analysis, controlled atmosphere, enzyme activity, Lilium longiflorum, storage conditions |
| Abstract:
Bulb-scales of Lilium longiflorum were stored for 180 days under different conditions of temperature (5 or 25 °C), atmosphere (ordinary or controlled, 50% N2 + 50% CO2, CA) and nutrient supply (none or the MS medium with 3 % sucrose). The fresh weight, water content, nutritional contents (total amino acid, total soluble protein, total sugar, starch), and the activities of glutamate dehydrogenase (GDH) and malate dehydrogenase (MDH) of the stored scales were measured to assess the effect of these factors on the stored scales. The viability of the explant was also assessed by the bulblet growth from the scales. Under the control condition (25 °C, ordinary air and no nutrient supply), all the nutritional contents and the MDH activity quickly decreased in the first 10 to 42 days. The bulblet growth from the stored scales was reduced according to the remaining nutritional contents and the MDH activity. It was noteworthy that the GDH activity decreased to one-tenth of the initial level in the first 21 days, and then increased to almost the initial level. Changes in fresh weight and water content were not related to the bulblet growth. On the other hand, low temperature, CA and nutrient supply effectively suppressed consumption of the nutritional contents in the bulb-scale. However, bulblet growth from stored scales did not always correspond to the remaining nutritional contents. Especially, CA effectively suppressed consumption most of the nutritional contents, but delayed the bulblet growth when the bulb-scale was stored for more than 42 days. | |
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