ISHS Acta Horticulturae 44: IX International Symposium on Fruit Tree Virus Diseases
APPLE LATENT VIRUSES: TRANSMISSION FROM HERBACEOUS HOST TISSUE TO MALUS INDICATOR PLANTS AND DISTRIBUTION IN APPLE BUDWOOD |
| Authors: | J.K. Uyemoto, R.F. Stouffer, R.M. Gilmer |
| Abstract:
Isolates of chlorotic leaf spot virus (CLSV) and stem grooving virus (SGV) were successfully transmitted from herbaceous host inocula to apple seedlings and Malus indicator budlings. Collectively, virus transmissions to Malus budlings were 14. 5, 2. 8, and 0%, respectively for approach grafts, tissue implantation, and sap inoculation. However, 22% of pre-darkened 10-day-old apple seedlings became infected following sap inoculation (Gilmer and Uyemoto, 1972). Infected plants were identified by assaying onto Chenopodium quinoa; diseased apple budlings and seedlings showed few, if any, diagnostic symptoms. Distribution of latent viruses in individual buds along a single budstick (comprised of one-and two-year old wood) was determined by indexing forced leaf tissue onto C. quinoa. Virus transmissions from one- and two-year-old buds were, respectively, 80 and 81% for CLSV; and 98 and 95% for SGV. | |
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