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ISHS Acta Horticulturae 439: III International Strawberry Symposium

SOMACLONAL VARIATION IN PLANTS REGENERATED BY ORGANOGENESIS FROM CALLUS CULTURE OF STRAWBERRY (FRAGARIA X ANANASSA)

Authors:   A.N. Popescu, V.S. Isac, M.S. Coman, M.S. Radulescu
Keywords:   N6-benzyladenine, 2,4-dichlorophenoxyacetic acid, in vitro culture, plant regeneration, somaclonal variants
Abstract:
Somaclonal variation was induced in strawberry plants regenerated from leaf and petiole-derived callus. Although the frequency of plant regeneration was generally low for all tested genotypes, a relatively high rate of variation was induced among regenerated somaclones by increasing the intervals for subcultures to 12 weeks, and also by transferring the calli, starting with the first subculture, to media containing combinations of BA (4.4, 13.3 or 22.2 μM) and 2,4-D (4.5 μM). In previous experiments these proved to be the most suitable for the maintenance of regeneration ability for a long period. The analysis of the origin, for all strawberry variants identified so far, suggests that both genotype and type of explant strongly influence the occurrence of somaclonal variation. Also, our results indicate that a relatively high number of variants could be obtained by controlling the factors involved in the maintenance of plant regeneration ability in strawberry calli for a long period. Several somaclones exhibiting useful variation in plant and fruit characteristics have been identified so far. A variant having a modified (white) colour of flesh for all fruits, induced from petiole-derived callus of cv. Gorella, is reported for the first time. Since most of the useful variations affecting plant vigour, fruit yield and runnering ability occurred in strawberry somaclones regenerated from leaf-derived callus, our results suggest that the type of variation in plants regenerated by organogenesis, is related to the type of explant.
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