ISHS Acta Horticulturae 439: III International Strawberry Symposium
IN VITRO SCREENING OF COLD RESISTANT STRAWBERRY CULTIVARS AND SEEDLINGS |
| Authors: | R. Rugienius, V. Stanys |
| Keywords: | Fragaria x ananassa, frost tolerance |
| Abstract:
Cold resistance of plants and proliferating structures in vitro have been studied within nine strawberry cultivars and seedlings of 4 combinations. Cultivars, known for their characters, were used as model in order to identify the possibilities for selection of cold-resistant seedlings in vitro. Microplants and proliferating structures were hardened for maximum 35 days at 2±0.5°C, frozen for 1–8 days at -8°C, -9°C, -10°C, -11°C, -12°C, -14°C in vitro in the media Murashige and Skoog with 1.5, 3, 6 or 9% sucrose. Explants were thawed at 2±0.5°C for 1–3 days. The degree of cold injury was evaluated according to 6-point scale (0–5). The best temperatures for freezing in vitro for differentiation of cultivars for cold resistance were -9°C to -11°C. Explants were more viable, whem time of hardening was longer. Viability of plants also depends on sucrose amount in the media. | |
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