ISHS Acta Horticulturae 436: I International Persimmon Symposium
EFFECT OF TYPES OF CYTOKININ USED FOR IN VITRO SHOOT PROLIFERATION OF JAPANESE PERSIMMON ON THE SUBSEQUENT ROOTING OF SHOOTS. |
| Author: | T. Tetsumura |
| Keywords: | BA, Diospyros kaki, Japanese persimmon, rejuvenation, rooting ability, subculture, zeatin |
| Abstract:
After establishing and proliferating onto the modified Murashige and Skoog (MS) medium supplemented with zeatin, the in vitro shoots of two Japanese persimmon cultivars were cultured on the same medium with 6-benzyladenine (BA) substituted for zeatin. Although the shoots of ‘Fuyu’ and ‘Hana Gosho’, which are hard to establish invitro with BA, stopped growing after transferring to the medium with BA, they started growing vigorously again when subcultured continuously with BA. During the initial period of subculturing with BA, they rooted poorly after rooting treatment, like those subcultured with zeatin only. After subculturing 17 times, however, the rooting ability of the shoots subcultured with BA increased with repeated subcultures. After subculturing 74≈76 times, finally, the shoots subcultured with BA showed a substantially higher rooting percentage (63≈65%) than those with zeatin (less than 30%). The mean number of the roots per rooted shoots subcultured with BA was 2.0≈4.5 after subculturing more than 40 times and most of the rooted shoots subcultured with zeatin only had one or two roots at all the rooting experiments. | |
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