ISHS Acta Horticulturae 433: I International Symposium on Edible Alliaceae
APPLICATION OF IN VITRO CULTURE TO ONION AND GARLIC FOR THE MANAGEMENT AND USE OF GENETIC RESOURCES AT GATERSLEBEN |
| Authors: | E.R. Joachim Keller, D. Lesemann |
| Keywords: | Allium, ethylene treatment, hybridization, in vitro bulbs, meristem culture, slow growth storage, virus elimination |
| Abstract:
The genebank Gatersleben and the Taxonomy department of the IPK maintain a large collection of Allium. In vitro culture is used for preservation of vegetatively propagated forms. Sucrose-induced bulblets were stored at different temperatures. Characterization of bulblet viability was performed by growth and ion efflux measurements. Dormancy was compared in material stored at + 3°C with or without warm preculture at + 25°C and with or without treatments with ethylene. Meristem culture was used for virus elimination. Elimination rates depended on the clone and vary from 15 to 58%. Hybrids from distant crosses of onion with various wild species were obtained by embryo rescue via ovary culture. They were verified by karyotype, isoenzyme, and RAPD analysis. | |
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