ISHS Acta Horticulturae 432: IX International Symposium on Virus Diseases of Ornamental Plants
CHARACTERIZATION OF A SINGAPORE ISOLATE OF HIBISCUS CHLOROTIC RINGSPOT VIRUS |
| Authors: | S.M. Wong, C.G. Chng, P.L. Chong, Y.H. Lee |
| Abstract:
A Singapore isolate of hibiscus chlorotic ringspot virus (HCRSV-S1) was obtained from Hibiscus rosa-sinensis L. exhibiting chlorotic leaf spots. Single lesion transfers were carried out on Chenopodium quinoa Willd. C. quinoa and kenaf were used for virus multiplication. Virus yield of 4 mg/100 g of infected leaves was achieved. Two rabbit antisera were raised against two virus bands obtained after sucrose density gradient centrifugation. Double-stranded RNA profile revealed three distinct bands. Virions were observed in cytoplasm of ultrathin sections from infected leaf tissues. The deduced coat protein amino acid composition was similar to that of HCRSV-F. | |
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