ISHS Acta Horticulturae 432: IX International Symposium on Virus Diseases of Ornamental Plants
IDENTIFICATION AND CHARACTERIZATION OF POTYVIRUSES OF ALSTROEMERIA SPP. |
| Authors: | I. Bouwen, R.&e.;A. van der Vlugt |
| Abstract:
Several viruses have been reported to infect Alstroemeria. The two most important ones are considered to be Alstroemeria mosaic (AIMV) and Alstroemeria streak virus (AISV). Both AIMV and AISV react in ELISA with a commonly used antiserum to AIMV. There are indications that potyviruses other than AIMV and AISV also occur in Alstroemeria. The present study was initiated to identify and characterise these potyviruses. A strategy was developed that is aimed at separation of these viruses and their subsequent individual characterization. It is based on obtaining isolated of single viruses by serial passages through a local lesion host followed by RT-PCR or immuno-capture RT-PCR to obtain virus-specific cDNA fragments. The nucleotide sequence of these cDNA fragments is compared to those of other known potyviruses present in the EMBL Database. Using this identification strategy four different potyviruses were identified, three of which were not known to occur in Alstroemeria. | |
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