ISHS Acta Horticulturae 432: IX International Symposium on Virus Diseases of Ornamental Plants
COMPARISON OF FOUR POTYVIRUS ISOLATES INFECTING AROID SPECIES |
| Authors: | C.G. Matthews, K.S. Milne, R.L.S. Forster, H.F. Neilson |
| Keywords: | Araceae, polymerase chain reaction, ELISA, dasheen mosaic potyvirus, taro feathery mottle potyvirus |
| Abstract:
Four potyvirus isolates believed to be dasheen mosaic potyvirus, the most frequently occuring virus to infect members of the Araceae, were obtained in Caladium, Colocasia, Xanthosoma and Zantedeschia from world-wide locations. Properties of these isolates including particle length, serological relatedness and genomic characteristics were compared. Serologically distinct strains of dasheen mosaic potyvirus were apparent amongst three of the isolates. Some differences in symptom expression occurred between these. The ability of cDNA, produced to the viral RNA, to bind with the primers used in the polymerase chain reaction were similar. An isolate from Colocasia esculenta (L.) Schott was not serologically related to the other isolates. Further isolates from C. esculenta also exhibited no relationship. The modal length was different from the other three isolates. The ability of cDNA, produced to the viral RNA, to bind with some of the primers used in the polymerase chain reaction also differed. This evidence led to the proposal that the isolate from C. esculenta was not dasheen mosaic potyvirus, this virus is tentatively named taro feathery mottle potyvirus. | |
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