ISHS Acta Horticulturae 432: IX International Symposium on Virus Diseases of Ornamental Plants
PARTIAL CHARACTERIZATION OF PELARGONIUM LINE PATTERN AND PELARGONIUM RINGSPOT VIRUSES |
| Authors: | G.R. Kinard, R.L. Jordan, S.S. Hurtt |
| Abstract:
Biological and serological properties of two viruses that infect geraniums, pelargonium line pattern virus (PLPV) and pelargonium ringspot virus (PelRSV), have been determined. Both viruses were established in and purified from C. quinoa. In electron microscopy 30–32 nm isometric particles were observed in purified preparations. Both viruses have a major protein species of 36–37 kDa. The double-stranded RNA patterns (dsRNA) elicited in infected hosts are similar for the two viruses, but can be distinguished on polyacrylamide gels. PLPV-infected C. quinoa contains two dsRNAs of 3.1 and 1.1 x 106 Mr, while PelRSV-infected C. quinoa contains two species of 3.3 and 1.2 x 106 Mr. Both of these patterns are distinct from those of known carmo-, diantho-, necro-, and tombusviruses. Antisera produced against a Netherlands isolate of PLPV reacts to PLPV isolates from the US and Denmark, but not with PelRSV. Antisera produced against PelRSV reacts to PelRSV, but not with PLPV isolates. PLPV and PelRSV are serologically distinct viruses. | |
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