ISHS Acta Horticulturae 432: IX International Symposium on Virus Diseases of Ornamental Plants
SIMPLE AND RAPID DETECTION OF ODONTOGLOSSUM RINGSPOT VIRUS FROM ORCHIDS BY GELATIN PARTICLE AGGLUTINATION TEST |
| Authors: | Y.J. LA, J.O. Park, H.W. Jung |
| Abstract:
The sensitivity and potential application of gelatin particle agglutination test(GPAT) for detecting odontoglossum ringspot virus(ORSV) in orchids were determined. The gelatin particles(3 um in diameter) were coated with the anti-ORSV IgG at a concentration of 20 ug/ml and reacted with several different concentrations of purified ORSV as well as varying dilutions of orchid leaf sap extracts. The GPAT detected as little as 0.1 ug/ml of ORSV when purified virus preparations were used. In crude leaf sap extracts from Cymbidium plants, the detectable dilution end point for the ORSV by GPAT was 10-4. The optimum dilution of leaf sap extract for the reliable detection of ORSV in Cymbidium and Cattleya plants by GPAT, however, was between 1:100 and 1:800. The GPAT required less than 2 hrs to detect ORSV in orchids. The GPAT was found to be a rapid, sensitive, reliable and simple serological method for detecting ORSV in orchids. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
