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ISHS Acta Horticulturae 431: Tospoviruses and Thrips of Floral and Vegetable Crops

DETECTION AND IDENTIFICATION OF TOSPOVIRUSES IN GREENHOUSES

Author:   M.L. Daughtrey
Abstract:
Of the greenhouse-grown flower samples submitted to our laboratory from 1989 to 1995, 5–15% annually have been identified as tospovirus infested. Since 1990, enzyme-linked immunosorbent assay (ELISA) has been used to differentiate tospoviruses. Only 18% of tospovirus-positive samples have been identified as tomato spotted wilt virus (TSWV); impatiens necrotic ringspot virus (INSV) predominates. Symptoms observed on 31 flower crops have included ringspots, stem cankers, pale white or yellow spots, necrotic spots, chlorotic mottle, leaf-base necrosis, necrotic or chlorotic line patterns, and stunting. These often mimic symptoms of other contagious diseases. A tospovirus has been detected in Diascia, Platycodon, and Scaevola, which have not been reported as hosts of tospoviruses.

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