ISHS Acta Horticulturae 431: Tospoviruses and Thrips of Floral and Vegetable Crops
BREEDING TRANSGENIC TOMATOES FOR RESISTANCE TO TOMATO SPOTTED WILT VIRUS AND CUCUMBER MOSAIC VIRUS |
| Authors: | C. Gonsalves, B. Xue, S.Z. Pang, R. Provvidenti, J.L. Slightom, D. Gonsalves |
| Abstract:
We have transferred the nucleocapsid protein gene of the lettuce isolate of tomato spotted wilt virus (TSWV-BL) into a tobacco mosaic virus (TMV) resistant tomato (Lycopersicon esculentum) line, (Geneva 80), via Agrobacterium tumefaciens. Kanamycin-resistant transgenic R0 lines were selfed and their progenies were resistant to the homologous TSWV-BL isolate. Transgenic R1 plants were also tested for their ability to resist infections by other tospoviruses. In addition, R2 lines were obtained from TSWV-BL-resistant R1 plants. One of these lines was crossed with a cucumber mosaic virus (CMV) resistant transgenic Geneva 80 line that is homozygous for the coat protein gene of CMV. Their progenies were resistant to TSWV and CMV. | |
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