ISHS Acta Horticulturae 431: Tospoviruses and Thrips of Floral and Vegetable Crops
DIAGNOSIS OF TOSPOVIRUSES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION |
| Authors: | R.J. Weekes, R.A. Mumford, I. Barker, K.R. Wood |
| Abstract:
Immunocaptured virus or RNA extracted from infected tissue, and primers corresponding to segments of the L genomic RNA, were used to identify tomato spot wilt virus (TSWV) by reverse-transcription polymerase chain reaction (RT-PCR). Primers corresponding to sections of the S genomic RNA species were used to detect impatiens necrotic spot virus (INSV). A primer mixture containing sequences for amplified segments of both the L and S viral genomic RNAs was used to detect and differentiate both TSWV and INSV in the same multiplex reaction mixture by producing a PCR product of characteristic size. A second set of universal primers containing sequences for amplification of a different section of the S RNA was also used to detect all tospoviruses tested. | |
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