ISHS Acta Horticulturae 430: VII International Symposium on Flowerbulbs
THE USE OF MORPHOGENIC SUSPENSION CULTURES FOR THE DEVELOPMENT OF A PROTOPLAST REGENERATION SYSTEM IN LILY |
| Authors: | I. Famelaer, M. Bordas, E. Baliu, E. Ennik, H. Meijer, J.M. van Tuyl, J. Creemers-Molenaar |
| Keywords: | Callus, picloram, 1-naphtylacetic acid, 6-benzylaminopurine, L. longiflorum, Asiatic, Oriental hybrid |
| Abstract:
The present study reports data on the development of a protoplast regeneration procedure in lily. Established morphogenic suspension cultures were obtained from callus cultures induced on mature embryos from crosses between cultivars of L. longiflorum. The effect on the frequency of protoplast division and microcolony formation of preculture conditions and origin of suspension cultures, composition of the protoplast isolation and culture media, anti-oxidants, an ethylene inhibitor, fetal calf serum, conditioned medium or a feeder culture and culture conditions are discussed. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
