ISHS Acta Horticulturae 430: VII International Symposium on Flowerbulbs
BULBLET REGENERATION IN VITRO FROM DIFFERENT EXPLANTS OF FRITILLARIA IMPERIALIS |
| Authors: | M. Witomska, A.J. Lukaszewska |
| Keywords: | micropropagation, Fritillaria imperialis, light, darkness, shoot segments |
| Abstract:
Trials to micropropagate Fritillaria imperialis cv. Rubra Maxima from different parts of the outdoor grown plants lifted in April were undertaken. Explants were excised from bulblets initiated at the shoot base, the lower leafless part of the shoot and the upper leafy part of the shoot. Shoot explants were prepared by cutting the shoot into 5 mm thick slices which were then cut in half. Rate of bulblet induction was evaluated after 1, 2 and 3 months of culture at 22°C on MS medium with 1 mg/l BA and 0.5 mg/l NAA. Half of the explants were cultured in darkness and half under fluorescent light for 16h per day. Scale segments regenerated loose callus and bulblets regenerated on only 4% of explants cultured in light. Shoot bases proved to be the least suitable material for micropropagation: 50% of explants in light and 76% in darkness died already after 1 month of culture. After three months in culture, 64% and 83% of explants excised from leafless shoot parts regenerated plantlets in light and darkness, respectively. Bulblet regeneration was most rapid and efficient on explants taken from leafy shoot segments, 84% regenerated in the light and 95% in darkness. Bulblet regeneration also occured in explants taken from receptacles and leaves formed above the inflorescence. | |
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