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ISHS Acta Horticulturae 430: VII International Symposium on Flowerbulbs

PROPAGATION OF TULIP IN VITRO

Authors:   A.-M. Kuijpers, M. Langens-Gerrits
Keywords:   STS, silverthiosulphate, PP333, paclobutrazol, Me-Ja, methyljasmonate, JA, jasmonic acid
Abstract:
A method for propagation of tulip in vitro is reported. Shoots were regenerated from stem explants placed on medium containing 5μM zeatin and 5μM a-naphthaleneacetic acid. However, about 90% of the structures formed under these conditions did not contain a meristem and could not be used for propagation. Meristem formation on stem explants was slightly improved by silverthiosulphate and strongly by paclobutrazol or methyljasmonate. The propagation rate under standard conditions was about 1.5 in ten weeks and increased after preculture of shoots in liquid medium.

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