ISHS Acta Horticulturae 430: VII International Symposium on Flowerbulbs
SELECTIVE AGENTS AND MARKER GENES FOR USE IN GENETIC TRANSFORMATION OF GLADIOLUS GRANDIFLORUS AND TULIPA GESNERIANA |
| Authors: | J.E. Chauvin, H. Hamann, J. Cohat, M. Le Nard |
| Keywords: | selective agents, marker genes, transformation, tulip, gladiolus |
| Abstract:
Successful plant transformation requires efficient regeneration and selection systems. In vitro development of tulip floral scape segments and gladiolus cormel slices was analyzed in the presence of kanamycin, hygromycin and phosphinothricin. Like most monocots, tulip and gladiolus were relatively insensitive to kanamycin even at high concentrations. Hygromycin was effective in controlling the regeneration of plantlets. The most effective agent was phosphinothricin. It totally inhibited regeneration for both tulip and gladiolus even at low medium concentration. In the greenhouse, we observed a strong necrotic effect after Basta spraying of the foliage. Endogenous  -glucuronidase activity was not detected in any gladiolus explants.
In contrast, this activity was present in some of the tulip explants even when sampling was done in situ. This preliminary study is very useful for choosing the plasmids containing the right constructions for further investigations on tulip and gladiolus transformation.
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