ISHS Acta Horticulturae 429: XIII International Symposium on Horticultural Economics
MASS PROPAGATION OF SYZYGIUM CUMINII FROM SELECTED ELITE TREES |
| Authors: | P.K. Roy, M. Rahman, S.K. Roy |
| Keywords: | Clonal propagation, In vitro culture, Syzygium cuminii |
| Abstract:
Multiple shoots were obtained from nodal explants of 10-year-old elite trees of Syzygium cuminii on Murashige and skoog's medium supplemented with 2.5 mgl-1 kinetin (Kn). Explants taken from in vitro proliferated shoots produced further multiple shoots when cultured on the same basal medium containing 2.5 mgl-1 Kn. Repeated subculture resulted in rapid shoot multiplication at an average rate of 10 shoots per subculture. Addition of 100 mgl-1 urea in the medium enhanced the number of shoots up to 60 per culture and incorporation of 100 mgl-1 casein hydrolysate (CH) and 15% (v/v) coconut milk (CM) to the medium increased the length of shoots. In vitro raised shoots rooted on half strength MS medium containing 0.5 mgl-1 each of indole -3- butyricacid (IBA),  -naphthaleneaceticacid (NAA) and indole-3-aceticacid (IAA). After transplanting in the field, 75% plantlets survived with uniform growth.
| |
Download Adobe Acrobat Reader (free software to read PDF files) | |
